Pablo Ricardo Arentes on Muck Rack

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CRISPR-Cas12a REC2 - NUC interactions drive target-strand cleavage and constrain trans cleavage.

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By Anthony Newman, Aakash Saha, Lora Starrs, Pablo Ricardo Arentes
| biorxiv.org
Abstract CRISPR−Cas12a effects RNA-guided cleavage of dsDNA in cis, after which it remains catalytically active and non-specifically cleaves ssDNA in trans. Native host-defence by Cas12a employs cis cleavage, which can be repurposed for the genome editing of other organisms, and trans cleavage can be used for in vitro DNA detection.
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