Panoraia Kotantaki on Muck Rack

Panoraia Kotantaki

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Articles

3D pentaculture model unveils malignant cell-driven macrophage polarization in high-grade serous ovarian cancer

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By Beatrice Malacrida, Samar Elorbany, Faisal Karim, Michail Sideris, Owen Heath, Ranjit Manchanda, Eleni Maniati, Frances R. Balkwill, Florian Laforêts, Rachel C. Bryan-Ravenscroft, Joash D. Joy, Panoraia Kotantaki, Sophie L. P. Skingsley
| Nature Verified
Abstract High-grade serous ovarian cancer (HGSOC) is characterized by a complex, immunosuppressive tumor microenvironment (TME) that contributes to poor clinical outcomes and resistance to therapy. To replicate the human TME in vitro, we develop a 3D pentaculture model incorporating five human cell types: malignant HGSOC cells and primary fibroblasts, mesothelial cells, adipocytes, monocytes. Monocytes differentiate into macrophages without exogenous cytokines in the pentacultures.
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