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Managing Autofluorescence in Spectral Flow Cytometry for Macrophage Identification in the Liver
AF autofluorescence cDC2 type 2 conventional dendritic cells KC Kupffer cell LAMs lipid-associated macrophages MASH metabolic dysfunction-associated steatohepatitis MASLD metabolic dysfunction-associated steatotic liver disease MdMs monocyte-derived macrophages moKCs monocyte-Kupffer cells 1 Introduction Autofluorescence (AF) in biological tissues is the natural emission of light from intra and extracellular molecules upon light absorption.
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