Patrick Klaus Jaeger

Request a detailed protocol We cut transversal (1 week) and longitudinal (3 weeks) cryosections (10 µm thickness) using a low-profile microtome blade (DB80 LX, BioSys Laboratories), collected them on a glass slide, and let them dry for 1 hr before storing them at –80°C until further use. Prior to staining, sections were air-dried for 30 min at RT and washed 3× with PBS for 5 min each. Then, sections were permeabilized and blocked with 3% BSA in PBS-T (PBS+0.1% Triton X) for 1 hr at RT.

Abstract Tendinopathies are debilitating diseases currently increasing in prevalence and associated costs. There is a need to deepen our understanding of the underlying cell signaling pathways to unlock effective treatments. In this work, we screen cell signaling pathways in human tendinopathies and find positively enriched IL-6/JAK/STAT signaling alongside signatures of cell populations typically activated by IL-6 in other tissues.