Paul Jerard Layug

Abstract OTX2 is a transcription factor and known driver in medulloblastoma (MB), where it is amplified in a subset of tumours and overexpressed in most cases of group 3 and group 4 MB. Here we demonstrate a noncanonical role for OTX2 in group 3 MB alternative splicing. OTX2 associates with the large assembly of splicing regulators complex through protein–protein interactions and regulates a stem cell splicing program.

1 INTRODUCTION Biological sex is defined by female or male based on chromosome composition (e.g., XX, XY), gonads (e.g., ovaries or testes), and gonadal hormones (e.g., estrogen or androgen). Intersex individuals are born with biological sex characteristics (e.g., reproductive anatomy and hormones) that do not fit exclusively to a male or female biological sex classification.