Pei Xuan Er

Abstract Creating complex multicellular kidney organoids from pluripotent stem cells shows great promise. Further improvements in differentiation outcomes, patterning, and maturation of specific cell types are, however, intrinsically limited by standard tissue culture approaches. We describe a novel full factorial microbioreactor array–based methodology to achieve rapid interrogation and optimization of this complex multicellular differentiation process in a facile manner.

Genome Medicine201911:3 https://doi.org/10.1186/s13073-019-0615-0 ©  The Author(s). 2019 Received: 24 September 2018 Accepted: 14 January 2019 Published: 23 January 2019 Abstract Background Human kidney organoids hold promise for studying development, disease modelling and drug screening. However, the utility of stem cell-derived kidney tissues will depend on how faithfully these replicate normal fetal development at the level of cellular identity and complexity.