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Pei-Yin Lim

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Articles

A nonstructural protein 1 capture enzyme-linked immunosorbent assay specific for dengue viruses

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By Thomas Loy, Angéline Rouers, Pei-Yin Lim, Appanna Ramapraba
| PLOS ONE
Discussion In this study, we characterized three newly isolated (A2, D6, and D8) and one previously published (Den3) human antibodies against NS1 and developed a dengue NS1 capture ELISA using these antibodies. To develop a DENV-specific NS1 capture ELISA, it is important that all antibodies fulfilled the following criteria: (1) recognize NS1 from multiple dengue serotypes; (2) bind to multimeric forms of NS1; and (3) have high affinity towards NS1.
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