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Petra Barackova

As seen in: Frontiers, JNeurosci
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Articles

Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes

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By Martin Klíma, Marek Ladislav, Petra Barackova, Emily Langore
| JNeurosci Verified
Analysis of the performance of three QD-based probes for tracking surface NMDARs in cultured neurons Our first step was to determine the most appropriate QD-based probe, establish the optimal experimental conditions, and then develop analytical tools to comprehensively analyze the surface trajectories of specific probe-labeled GluN subunits in cultured hippocampal neurons, including the most accurate method for differentiating between synaptic and extrasynaptic regions.
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The Extracellular Domains of GluN Subunits Play an Essential Role in Processing NMDA Receptors in the ER

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By Cesar Mattei, Alasdair Gibb, Gabriela Popescu, Petra Barackova
| Frontiers
Introduction N-methyl-D-aspartate receptors (NMDARs) are ionotropic glutamate receptors that play an essential role in mediating excitatory neurotransmission (Traynelis et al., 2010; Vieira et al., 2020). NMDARs are heterotetramers comprised of two GluN1 (with eight splice variants) and two GluN2 (GluN2A through GluN2D) and/or GluN3 (GluN3A and GluN3B) subunits (Paoletti, 2011; Perez-Otano et al., 2016).
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