Figure 6Asprosin Uses the cAMP Second-Messenger System and Activates PKA in the Liver(A) Liver cAMP level was measured 15 min after a single 30-μg dose of subcutaneous recombinant asprosin or GFP in mice that had been subjected to a 2-hr fast prior to injection (n = 6 in each group).(B) Liver PKA activity was measured in mice from (A).(C) Immunoblot analysis for phosphorylated PKA catalytic subunit or for phosphorylated serine/threonine PKA substrate was performed on liver lysates from mice...