Pradeep Rajan

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2. Materials and Methods 2.1. Cell Models Human HCC cell lines Hep3B (HB-8064) and SNU475 (CRL-2236) from ATCC (Manassas, VA, USA), free of mycoplasma contamination (RT-PCR kit, from MycoAlert PLUS Mycoplasma Detection Kit, Lonza, Rockland, MD, USA), underwent 2-5 passages prior to experimental use. Hep3B cells were grown in culture with high-glucose DMEM media supplemented with 10% FBS and 1% Penicillin/Streptomycin.