Propagation Changchun

Sample preparation The samples were washed and dried at 38°C until a constant weight was achieved, then main roots were separated and pulverized into powder, and passed through a 60-mesh sieve. A quantity of 0.1 g accurately weighed fine powder was extracted with 10 mL of 70% (v/v) methanol in an ultrasonic water bath for 60 min (Wang et al., 2019). The supernatant of extract was filtered through a syringe filter (0.22 μm) before liquid chromatography mass spectrometry system analysis.