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Proteomics Core UCCTs

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Articles

Endothelial proteomic response to Dirofilaria repens somatic antigens and the Dr20/22 protein reveals parasite-driven modulation of angiogenesis

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By Zoonotic Diseases, Centro Nacional de Microbiología, Proteomics Core UCCTs
| TechTrends
Antigen preparation Adult DrSA were prepared as described previously, with some modifications [20]. In brief, three adult D. repens worms extracted from a human subcutaneous nodule were washed in 1× phosphate-buffered saline (PBS), macerated at 4 °C, and sonicated in PBS pH 7.2 with five cycles of 70 kHz (30 s each cycle).
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