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Droplet digital PCR (ddPCR) using FFPE DNA to assess methylation status of MGMT gene among patients with IDH mutant astrocytoma and IDH wild-type glioblastoma
Abstract MGMT promoter methylation analysis in formalin-fixed paraffin-embedded (FFPE) tissues can be challenging since the DNA obtained is often fragmented. Bisulfite conversion, which is essential to determine methylation status, further degrades DNA.
Diagnostic utility of droplet digital PCR to detect TERT promoter mutations among glioblastoma samples using 7-deaza-dGTP
Diagnostic utility of droplet digital PCR to detect TERT promoter mutations among glioblastoma samples using 7-deaza-dGTP Rajadurai Abarna1, Rachel Nancy1, Ari Chacko2, Geeta Chacko1, http://orcid.org/0000-0002-7964-417XRekha Pai1 1 Pathology, Christian Medical College, Vellore, Tamil Nadu, India 2 Division of Neurosciences, Christian Medical College, Vellore, Tamil Nadu, India Correspondence to Dr Rekha Pai, Pathology, Christian Medical College Vellore, Vellore, Tamil Nadu, India;...
Diagnostic utility of droplet digital PCR to detect TERT promoter mutations among glioblastoma samples using 7-deaza-dGTP
Diagnostic utility of droplet digital PCR to detect TERT promoter mutations among glioblastoma samples using 7-deaza-dGTP Rajadurai Abarna1, Rachel Nancy1, Ari Chacko2, Geeta Chacko1, http://orcid.org/0000-0002-7964-417XRekha Pai1 1Pathology, Christian Medical College, Vellore, Tamil Nadu, India 2Division of Neurosciences, Christian Medical College, Vellore, Tamil Nadu, India Correspondence to Dr Rekha Pai, Pathology, Christian Medical College Vellore, Vellore, Tamil Nadu, India;...
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