Ralf-Ingo Ernestus

Abstract Organotypic brain slice cultures are well-established and valuable three-dimensional (3D) models in neuroscience, particularly for cancer research. However, many existing 3D models rely on adult animals or are restricted to specific brain regions, such as the hippocampus. To address these limitations, we established a whole, large-format brain slice model that preserves the complex cellular architecture of the brain and its diverse cell populations.

Keywords: Sporadic vestibular schwannoma next generation sequencing (NGS) pathogenic variants genotype phenotype Introduction Vestibular schwannoma are benign tumors of the vestibular nerve in the cerebellopontine angle. Most of them arise sporadically and only about 5% grow in patients with neurofibromatosis type 2 (NF2), a genetic disorder with an inherited inactivating variant of the NF2 gene, which codes for the tumor suppressor Merlin.