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Articles by Raphaël Collot
IGSF11-VISTA is a critical and targetable immune checkpoint axis in diffuse midline glioma
Keywords diffuse midline glioma spatial multi-omics profiling cancer-immune landscape microglia-cancer interaction IGSF11-VISTA immune checkpoint Introduction Diffuse midline glioma (DMG) is a highly aggressive pediatric brain tumor lacking curative treatments.1,2,3 DMG is commonly characterized by a histone H3 lysine 27-to-methionine (H3K27M) mutation, predominantly the H3.3K27M variant.4 This K27M mutation is often accompanied by mutations in oncogenes and tumor suppressor genes, with TP53...
BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment
eLife Assessment This is a useful tool for code-less analysis of patterns in cell migratory behaviours in vivo using intravital microscopy data and allows correlation with spatial features of the tumour microenvironment. There is a clear need for these tools to make quantitative analysis, comparison and interpretation of complex cell tracking data more accessible and solid evidence is provided of its applicability to tracks generated by both proprietary and open tracking software.
BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment
Abstract Intravital microscopy (IVM) enables live imaging of animals at single-cell resolution, offering essential insights into cancer progression. This technique allows for the observation of single-cell behaviors within their natural 3D tissue environments, shedding light on how genetic and microenvironmental changes influence the complex dynamics of tumors.
BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment
Abstract Intravital microscopy (IVM) enables live imaging of animals at single-cell level, offering essential insights into cancer progression. This technique allows for the observation of single-cell behaviors within their natural 3D tissue environments, shedding light on how genetic and microenvironmental changes influence the complex dynamics of tumors.
Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D - Nature Protocols
Abstract Revealing the 3D composition of intact tissue specimens is essential for understanding cell and organ biology in health and disease. State-of-the-art 3D microscopy techniques aim to capture tissue volumes on an ever-increasing scale, while also retaining sufficient resolution for single-cell analysis. Furthermore, spatial profiling through multi-marker imaging is fast developing, providing more context and better distinction between cell types.
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