Regitze Lund Nielsen on Muck Rack

Regitze Lund Nielsen

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Articles

Skumtennisstævne i Nr. Broby Hallen

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By Regitze Lund Nielsen
| Ugeavisen
Annonce
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A method for separating bacterial cultures into single cells and defined size fractions of biofilm aggregates

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By Thomas Bjarnsholt, Tim Holm Jakobsen, Mads Lichtenberg, Regitze Lund Nielsen
| biorxiv.org
Abstract In vitro microbiological experiments that aim to describe differences between planktonic and biofilm aggregate populations are challenging since liquid batch cultures contain a mix of both. Here, we present a simple method for fractioning a bacterial liquid batch culture into aggregates and single cells. Stackable cell strainers with mesh sizes of 30 um and 10 um were used to filtrate 6 day old batch cultures of Pseudomonas aeruginosa to produce size fractions of 0-10 um and >30 um.
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