Renée Lynn Corpuz

Abstract Obligate microbial symbioses are often characterized by streamlined biosynthetic pathways and reduced genomes. The evolutionary process of this reduction first involves an increase in the abundance of non-functional coding genes (pseudogenes) followed by their removal. The olive fruit fly (Bactrocera oleae) harbors an extracellular symbiotic gut bacterium Candidatus Erwinia dacicola, which is crucial to its usage of fruit from the olive genus Olea as a larval food source.

Abstract By coupling chromatin conformation capture (3C) with PacBio HiFi long-read sequencing, we have developed a new method (CiFi) that enables analysis of genome interactions across repetitive genomic regions with low-input requirements. CiFi produces multiple interacting concatemer segments per read, facilitating genome assembly and scaffolding.

Abstract The West Indian fruit fly, Anastrepha obliqua, is a major pest of mango in Central and South America and attacks more than 60 species of host fruits. To support current genetic and genomic research on A. obliqua, we sequenced the A. obliqua genome using high-fidelity (HiFi) long-read sequencing. This resulted in a highly contiguous contig assembly with 90% of the genome in 10 contigs. The contig assembly was placed in a chromosomal context using synteny with a closely related species, A.