Rikinari Hanayama

Abstract Cells release intraluminal vesicles in multivesicular bodies as exosomes to communicate with other cells. Although recent studies suggest an intimate link between exosome biogenesis and autophagy, the detailed mechanism is not fully understood. Here we employed comprehensive RNA interference screening for autophagy-related factors and discovered that Rubicon, a negative regulator of autophagy, is essential for exosome release.

Abstract Methods that enable specific and sensitive quantification of small extracellular vesicles (sEVs) using flow cytometry are still under development. Aggregation or adsorption of antibodies causes sub-nano sized particles or non-specific binding and largely affects the results of flow cytometric analysis of single sEVs. Comparison of control IgG and target-specific IgG is inappropriate because they have different characters.

Newsletter | February 11, 2022 By Roberta Duncan, VP, mRNA Program Lead, Seqirus While the first paper to show that mRNA could be used as a vaccine was published in 1990, its efficacy in a Phase 3 clinical trial was not determined until 2020. Now, as we look to the future of vaccine development, mRNA has become a priority in many vaccine research programs. Among other benefits, the tech can be moved into clinical trials more rapidly than other platforms.