Riley Harrison

The Cas9 nuclease has become central to modern methods and technologies in synthetic biology, largely due to the ease with which it can be targeted to specific DNA loci via guide RNAs (gRNAs). Reports vary widely on the actual specificity of this targeting, with some studies observing 60% of gRNAs possessing no activity against the genome, yet an assumption persists within the E. coli community that inactive gRNAs are rare.

Investment Info: Small multi-family (2-4 units) buy & hold investment in Plumerville. Purchase price: $283,000 Cash invested: $19,000 This investment was purchased as a single family home with an attached in-law apartment. For a short time a have lived in the apartment and rented the home. The property needed some cosmetic work that I have done myself. I was able to negotiate and get a $7000 check at closing for a new roof! Home has already gone up about $30,000 in value since bought.

Abstract The Cas9 nuclease has become central to modern methods and technologies in synthetic biology, largely due to the ease in which it can be targeted to specific DNA loci via guide RNAs (gRNAs). Reports vary widely on the actual specificity of this targeting, with some studies observing 60% of gRNAs possessing no activity against the genome, while there is a general assumption in the E. coli community that inactive gRNAs are rare.