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Articles by Robert Maple
In vivo properties of Arabidopsis FCA condensates involved in RNA 3′ processing
Abstract Our understanding of the functional requirements underpinning biomolecular condensation in vivo is still relatively poor. The Arabidopsis RNA binding protein FCA is found in liquid-like nuclear condensates that function in RNA 3′ processing, promoting proximal polyadenylation at many targets in the Arabidopsis genome. To further understand the properties of these condensates in vivo we used single particle tracking experiments on FCA stably expressed at endogenous levels in plant nuclei.
A CPF-like phosphatase module links transcription termination to chromatin silencing
Highlights • APRF1, a homolog of Swd2/WDR82, is required for FCA-mediated FLC repression • APRF1 in a CPF-like phosphatase module with TOPP4 (Glc7/PP1) and LD (Ref2/PNUTS) • Loss of APRF1 leads to transcriptional readthrough and RNA Pol II hyperphosphorylation • APRF1 activity links co-transcriptional processing with chromatin remodeling Summary The interconnections between co-transcriptional regulation, chromatin environment, and transcriptional output remain poorly understood.
Proximal termination generates a transcriptional state that determines the rate of establishment of Polycomb silencing
Highlights • Transcription, co-transcriptional processing, and chromatin state are tightly linked • Transcription-coupled repression links H3K4me1 removal to proximal polyadenylation • Mathematical modeling reveals how this mechanistically links to silencing by PRC2 • More effective transcription-coupled repression enables faster epigenetic silencing Summary The mechanisms and timescales controlling de novo establishment of chromatin-mediated transcriptional silencing by Polycomb repressive...
Modular structure of RNA 3′ processing condensates involving the Arabidopsis RNA binding protein FCA
Abstract The Arabidopsis RNA binding protein FCA is found in liquid-like nuclear condensates and promotes proximal polyadenylation of specific nascent transcripts. To understand the in vivo interactions required for these condensates we used single-particle tracking experiments on FCA expressed at endogenous levels in live cells.
Proximal termination generates a transcriptional state that determines the rate of establishment of Polycomb silencing
Abstract Chromatin-mediated transcriptional silencing by Polycomb Repressive Complex 2 (PRC2) is critical for gene regulation in development and environmental responses. However, the mechanism and timescales controlling de novo establishment of PRC2 silencing are unclear. Here, we investigate PRC2 silencing at Arabidopsis FLOWERING LOCUS C (FLC), known to involve co-transcriptional RNA processing, histone demethylation activity, and PRC2 function; but so far not mechanistically connected.
A CPF-like phosphatase module links transcription termination to chromatin silencing
Abstract The interconnections between co-transcriptional regulation, chromatin environment and transcriptional output remain poorly understood. Here, we investigate the mechanism underlying RNA 3 processing-mediated Polycomb silencing of Arabidopsis FLOWERING LOCUS C (FLC). We show a requirement for APRF1, a homologue of yeast Swd2 and human WDR82, known to regulate RNA Pol II during transcription termination.
The CPSF phosphatase module links transcription termination to chromatin silencing
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A transposon surveillance mechanism that safeguards plant male fertility during stress
Abstract Although plants are able to withstand a range of environmental conditions, spikes in ambient temperature can impact plant fertility causing reductions in seed yield and notable economic losses1,2. Therefore, understanding the precise molecular mechanisms that underpin plant fertility under environmental constraints is critical to safeguarding future food production3.
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