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Materials & Methods Ethics statement All experimental procedures imposed on animals were approved by the Animal Use Ethics Committee of Federal University of Rio Grande (CEUA–FURG) (project 23116.006908/2017—81), and all procedures used were consistent with the established guidelines of the National Council for the Control of Animal Experimentation (CONCEA). Animal care Two-year-old black drum males (Pogonias courbina) were used in this study (38.3 ± 1.8 cm).
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