Ronja S Adam

LJS, RSA and LFM contributed equally. LV and NL contributed equally. Contributors LJS, RSA, LFM, NL and LV conceptualised the project. LJS, NL, LEN, AL, SE and OB performed in vitro experiments. LJS visualised the data. RSA and LFM performed data analysis and visualisation of GRO-seq and RNA-seq with support from DAZ and JK. RSA analysed and visualised the CRISPRi screen. LJS, KJL, SMvN, NL and LV designed in vivo experiments. LEN, AL, NEdG and SMvN performed in vivo experiments.

Materials and methods Colon cancer cell lines (Sanger) were cultured in Dulbecco’s Modified Eagle Medium/F12 (LoVo, HT55, SW1463, LS180, CL-40, HuTu-80, HCT116, T84, SW48, Caco-2) or Roswell Park Memorial Institute (LS411N, LS1034, MDST8, COLO320-HSR) containing 10% fetal calf serum (Life Technologies), penicillin and streptomycin.