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Ross Joaquin Hatlen on Muck Rack

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Detection of Podosphaera macularis in Air Samples by Quantitative PCR

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By David Gent, Timothy Miles, Nanci Adair, Ross Joaquin Hatlen
| apsjournals.apsnet.org
Detection and quantification of pathogen propagules in the air or other environmental samples is facilitated by culture-independent assays. We developed a quantitative PCR assay for the hop powdery mildew fungus, Podosphaera macularis, for detection of the organism from air samples. The assay utilizes primers and a TaqMan probe designed to target species-specific sequences in the 28S large subunit (LSU) of the nuclear ribosomal rDNA.
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