Ryou Makino

Conflicts of Interest The authors declare no conflicts of interest. Data Availability Statement The data that support the findings of this study are not publicly available due to privacy and ethical restrictions. De-identified data may be made available from the corresponding author upon reasonable request. References 1, , and , “Fine-Needle Aspiration Cytology for Parotid Tumors,” Life 12 (2022): 1897.

Despite the growing attention on the use of cytology samples for genetic analysis, the impact of the time from sample collection to fixation, referred to as ‘cold’ ischemic time, has not been sufficiently studied. Therefore, we investigated the quality of nucleic acids prior to fixation in body cavity fluid samples, focusing on the benign/malignant status, differences in collection methods and tumour cell content.