Highlights • AAV1 and AAV6 robustly transduce mouse zygotes • CRISPR-READI uses RNP electroporation and AAV donors for efficient mouse genome editing • CRISPR-READI enables complex genome engineering with HDR donor lengths up to 4.9 kb Summary Genetically engineered mouse models harboring large sequence insertions or modifications are critical for a wide range of applications including endogenous gene tagging, conditional knockout, site-specific transgene insertion, and gene replacement;...
