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Salini Scaria

As seen in: Frontiers, BMC Cancer
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Articles

Label–free quantitative urinary proteomics for non-invasive biomarker discovery in endometrial cancer

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By Abdulaziz City, Salini Scaria
| Frontiers
Abstract Background: Endometrial cancer (EC) is a growing global healthcare concern. The diagnosis of EC involves invasive techniques. There is a pressing need for reliable, non-invasive screening methods utilizing biomarkers that diagnose these patients. Methods: We conducted a case–control study consists of 40 women including 20 EC (cancer group) and 20 controls (control group) recruited from King Khalid University Hospital, King Saud University.
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Plasma-based proteomic profiling identifies the distinct regulation of proteins in hyperplasia and endometrial cancer - BMC Cancer

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By Abdulaziz City, Salini Scaria
| BMC Cancer Verified
To assess the differential protein expression among the EC, Hy and CO groups (34 samples from 17 gels), we performed 2D-DIGE and MALDI-TOF MS. Proteins were separated on IPG strips (pH 3–11) in the first dimension, followed by 12.5% PAGE in the second dimension. The gels were scanned with a Sapphire Biomolecular Imager (Azure Biosystems, Dublin, OH, USA) and digitalized via the image analysis software Sapphire Capture system (Azure Biosystems, Dublin, OH, USA).
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