Sanzeng Zhao

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The recent advancements in developing the CRISPR/Cas9 system and various derivative tools (e.g., base editors) have accelerated basic plant science research and crop improvement by creating multiple types of genetic variations (Li et al., 2023a). However, the use of Cas9 protein is frequently limited by the requirement of G/C-rich protospacer-adjacent motif (PAM) sequences, especially in triticeae plants, many of which are important food and forage crops carrying large and complex genomes.