Sapna K Deo

2.5. Case Study Here, we provide a practical example of our methodology, applying each step in sequence. Initially, we randomly selected 1600 tetrapeptides, i.e., 1% of the sample pool. These are subjected to a flexible docking procedure against the WNV receptor using AutoDock CrankPep (ADCP) a tool featured in ADFR software specifically designed for peptide-protein docking [29]. This tool is capable of automatically generating 3-D structures of peptides.

Here we describe the design of a bioluminescent stem-loop probe for the sensitive detection of HIV-1 spliced RNA. In this study, we employed Gaussia luciferase (GLuc), a bioluminescent protein that has several advantages over other bioluminescent proteins, including smaller size, higher bioluminescent intensity, and chemical and thermal stability. GLuc was chemically conjugated to the DABCYL-modified stem-loop probe (SLP) and was purified with a 2-step process to remove unconjugated GLuc and SLP.