Sara Cook

Established F0 CRISPR screening approach in zebrafish to test the role of developmental and disease gene functions in ENS neuronal development, enteric progenitor cell migration, and intestinal transit. As a proof-of-concept, targeting the known ENS regulator genes sox10, ret, or phox2bb phenocopied known ENS phenotypes with high efficiency using F0 CRISPR approaches. We identified five potential new regulator genes of ENS neurogenesis, some of which affected intestinal transit.