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The difference in calcium sensitivity could instead be due to differences in the coupling efficiency of Ca2+ entry to DA or GABA release. We compared the relative Ca2+ coupling distance of DA or GABA release using exogenous membrane-permeable Ca2+ chelators BAPTA-AM and EGTA-AM, which act through fast and slow binding kinetics, respectively, to determine whether sources of Ca2+ are tightly or loosely coupled to transmitter release ( ).
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