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Savannah Barkdull on Muck Rack

Savannah Barkdull

As seen in: Nature, BioRxiv
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Articles

Single cell analysis of developing Merkel cells reveals the emergence of non-coding RNA biotypes as a hallmark of terminal differentiation - Cell Death & Differentiation

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By Savannah Barkdull, Patrick Hallaert, Michael Kelly, Matthew W. Kelley
| Nature Verified
Abstract RNA processing generates diverse protein-coding and non-coding transcripts, yet RNA biotype diversity during cellular differentiation is not well characterized. Merkel cells (MCs) are cutaneous mechanosensors. We analyzed full-length transcripts of FACS-sorted single mouse MCs at all stages of development and discovered that their terminal differentiation was accompanied by an emergence of non-coding transcripts associated with genes related to MC function.
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Estimating and correcting index hopping misassignments in single-cell RNA-seq data

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By Lingling Miao, Loren Collado, Savannah Barkdull, Yoshine Saito
| BioRxiv
Abstract Index hopping causes read assignment errors in data from multiplexed sequencing libraries. This issue has become more prevalent with the widespread use of high-capacity sequencers and highly multiplexed single-cell RNA sequencing (scRNA-seq) libraries. We conducted deep, plate-based scRNA-seq on a mixed population of mouse skin cells. Analysis of transcriptomes from 1152 cells identified four distinct cell types.
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