Sayed Mehedi Azim

Abstract Accurate segmentation and identification of hippocampal subregions are essential for understanding spatial memory, neuronal plasticity, and disease-related alterations in brain architecture. While fluorescent immunohistochemistry (IHC) enables detailed visualization of subregion-specific molecular markers, automated segmentation and classification remain challenging due to staining variability, morphological complexity, and low signal-to-noise ratios.

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