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Measuring the Degree of Labeling of Antibody-Dye Conjugates with a Single-Molecule-Sensitive Digital Flow Cytometer
Abstract Click to copy section linkSection link copied! Antibody-dye conjugates are an essential tool in biomedical research and are widely used in flow cytometry, immunofluorescence imaging, and ELISAs. The degree of labeling (DOL), often referred to as the F/P (fluorophore-to-protein) ratio, which is the number of dyes per antibody, is a fundamental characteristic of antibody-dye conjugates that impacts conjugate performance (emission intensity, affinity, solubility/aggregation) and the...
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