A Fraction of Meg3 RNA Is Retained and Overlaps the Maternal Dlk1 Gene (in cis) To explore imprinted gene expression at the Dlk1-Dio3 domain (Figure 1Figure 1A), we generated mice that were hybrid between C57BL6/J and M. m. molossinus inbred strain JF1. Total RNAs from neonatal (P0) brain tissues were analyzed (Figure 1Figure 1B). Dlk1 expression was high in hypothalamus (HT), cortex (C), and cerebellum (CB). Much lower expression was apparent in hippocampus (HC).
