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Seo Hyun Lim

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Biosynthesis of polyhydroxyalkanoates from sucrose by recombinant Pseudomonas putida KT2440

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By Materials Science KOREA, Eun Seo Lee, Dojin Kim, Seo Hyun Lim
| Wiley Online Library Verified
A sucrose-utilization pathway was developed in Pseudomonas putida using sacC from Mannheimia succiniciproducens, which encodes a β-fructofuranosidase that hydrolyzes sucrose into glucose and fructose. Excretion of β-fructofuranosidase into the culture medium was confirmed via western blot analysis. In nitrogen-limited cultivation, P. putida expressing SacC produced 10.52 wt% medium-chain-length polyhydroxyalkanoate (MCL-PHA), while P.
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