Serkan Sayin

Abstract Bacterial colonization of cancer tumors is widespread. While the clinical significance of intratumor bacteria is widely investigated, the dynamics of tumor colonization remain underexplored. We monitored bacterial population dynamics in murine tumors after introducing genetically barcoded Escherichia coli cells by intravenous or intratumor injections.

Since the advent of microscopy in the 17th century, it has become well established that organisms are divided into tissues made up of different types of cells, with cells of the same type typically performing the same role. This simplifies the task of understanding a biological system immensely, as there are many fewer cell types than individual cells (Masland, 2001).

We repeated the genetic screen in two formats: We repeated the screen with the same collection of knockout strains and we repeated the screen with independent strain collection (knockout strains that were independently cloned). The results from these two completely independent biological replicates are presented on Figure 1—figure supplement 1D. The results (resistance/sensitivity) from both these validation screens were highly correlated with the original screen.