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Servicio Virus Oncogénicos

As seen in: PLOS
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Articles

Human papillomavirus genotyping using next generation sequencing (NGS) in cervical lesions: Genotypes by histologic grade and their relative proportion in multiple infections

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By Servicio Virus Oncogénicos, Joan Valls, Jorge Alejandro Basiletti
| PLOS
Results One hundred thirty-seven cervical samples were sequenced by NGS and bioinformatically analysed for HPV detection. When using a cut-off value of 50 reads, HPV types in plasmid samples and HPV negative cell lines were correctly detected and typed with NGS at different DNA amounts (5, 50 and 500 copies/5μL for HPV16 and HPV18; 50 and 500 copies/5μL for the other HPV types). Therefore, a threshold of 50 reads was set for positive samples.
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Distribution of human papillomavirus genotypes by severity of cervical lesions in HPV screened positive women from the ESTAMPA study in Latin America

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By Armando Baena, Joan Valls, Rita Mariel Correa, Servicio Virus Oncogénicos
| PLOS
Loading metrics Open Access Peer-reviewed Research Article Abstract The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance of the other HR-HPV differs slightly by geographical region. Here, we studied the HPV genotype distribution of HPV positive Latin American (LA) women by histological grade, in a sub-cohort from the ESTAMPA study; we also explored the association of age-specific HPV genotypes in severe lesions.
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