Shauni De Vriese

1 Introduction Atopic dermatitis (AD) is a complex inflammatory skin disease characterized by severe pruritus, impaired epidermal barrier, and immune dysregulation. While several phenotypes have been classified based on clinical characteristics, there is no established consensus on AD endotypes. However, specific immune pathways and their associated cytokines or biomarkers have been identified [1-3].

Key Points We describe a highly effective and accessible approach for the purification of human serum IgE. This method is useful for experimental models in the field of allergy and clinical immunology. It is possible that this method can be scaled up to larger volumes if needed. Abstract For Ab purification, high-affinity chromatography is commonly used. This technique results in high-purity Abs, but it requires highly specific knowledge and equipment.