Shenfei Sun

Abstract Lgr5+ stem cells play crucial roles in maintaining intestinal epithelial cell homeostasis. However, the cellular heterogeneity and underlying regulatory programs of Lgr5+ small intestinal stem cells (ISCs) remain elusive. In this study, we profiled gene expression and chromatin accessibility of Lgr5+ ISCs at single-cell resolution to gain a deeper understanding of the lineage specification and early fate determining mechanisms.

Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description imm13957-sup-0001-FigureS1.pdfPDF document, 520.2 KB Figure S1. (A) Absolute body weights of mice (Ad Figure 1A). (B) Sirius Red staining of mouse lungs (WT and ALK3ΔCD11c) at d14 after Bleo. (C) Quantification of fibrosis and airspace. (D) Immune cells in BAL at d14 after Bleo. (E) Lung function at d14 after Bleomycin. (F) Absolute body weights of mice (Ad Figure 1E).

Abstract Postnatal respiration requires bulk formation of alveoli that produces extensive surface area for gas diffusion from epithelium to the circulatory system. Alveolar morphogenesis initiates at late gestation or postnatal stage during mammalian development and is mediated by coordination among multiple cell types.