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Shir-Liya Dadon on Muck Rack

Shir-Liya Dadon

As seen in: BioRxiv
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Articles

Expanding single-cell toolbox with a cost-effective full-length total RNA droplet-based sequencing technology

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By Xue Sun, Dena Ennis, Shir-Liya Dadon, Wenpeng Fan
| BioRxiv
Abstract sc-rDSeq is a scalable, full-length total RNA droplet-based technology that captures both polyadenylated and non-polyadenylated RNAs, including histone-RNAs, small and long non-coding RNAs, and enhancer RNAs. It achieves a tenfold increase in UMIs per cell over inDrops while remaining simple and cost-efficient. Applied to lung cancer cells, sc-rDSeq revealed hidden heterogeneity, divergent signaling pathways, and non-polyA RNA variations undetectable with 3' end-based methods.
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