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Transcriptome Sequencing Reveals Survival Strategies and Pathogenic Potential of Vibrio parahaemolyticus Under Gastric Acid Stress
2. Materials and Methods 2.1. The Selected Experimental Strains and Culture Methods V. parahaemolyticus VPE5 (tlh positive, tdh and trh negative), VPE7 (tlh and tdh positive, trh negative), VPE27 (tlh positive, tdh and trh negative), and VPE28 (tlh and tdh positive, trh negative) environmental strains were used in this study [11]. V. parahaemolyticus frozen at −80 °C was inoculated onto Thiosulfate–Citrate–Bile salt–Sucrose (TCBS) agar and cultured at 37 °C for 9 to 14 h.
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