Abstract Repetitive transcranial magnetic stimulation (rTMS) is well-established for its ability to induce synaptic plasticity. However, its impact on structural and functional remodeling within stimulated networks remains not well-understood. To bridge the gap between cellular and network-level effects, we investigated plasticity mechanisms in entorhino-hippocampal tissue cultures subjected to a clinically approved 600-pulse intermittent theta burst stimulation (iTBS600) protocol.