Shuaijiang Jin

Chemical cross-linking enables rapid identification of RNA-protein and RNA-nucleic acid inter- and intramolecular interactions. However, no method exists to site-specifically and covalently cross-link two user-defined sites within an RNA. Here, we develop RNA-CLAMP, which enables site-specific and enzymatic cross-linking (clamping) of two selected guanine residues within an RNA.

Author Correction: Traceless native chemical ligation of lipid-modified peptide surfactants by mixed micelle formation Correction to: Nature Communications https://doi.org/10.1038/s41467-020-16595-w, published online 3 June 2020. This article contains an error in Fig. 1b, in which the Dap(PhCn) amino acid was missing a methylene group between the nitrogen and the aromatic ring. The correct version of Fig. 1b is: The error has been corrected in the PDF and HTML versions of the article.

Abstract Biology utilizes multiple strategies, including sequestration in lipid vesicles, to raise the rate and specificity of chemical reactions through increases in effective molarity of reactants. We show that micelle-assisted reaction can facilitate native chemical ligations (NCLs) between a peptide-thioester – in which the thioester leaving group contains a lipid-like alkyl chain – and a Cys-peptide modified by a lipid-like moiety.