Abstract Objective: This study aims to explore whether gastrodin (Gas) combined with donepezil (Don) has a neuroprotective effect on Alzheimer’s disease (AD) mice by regulating the AMPK/PGC-1α/SIRT3 signaling axis. Methods: HT22 cells were transfected with SIRT3 siRNA or control siRNA. Cells were then treated with the AMPK inhibitor, Don, Gas, or their combination. Subsequently, HT22 cells were treated with Amyloid beta 1–42 (Aβ 1–42) to induce an in vitro AD model.
