Shuoliang Wang

ORIGINAL RESEARCH article Volume 16 - 2025 | https://doi.org/10.3389/fmicb.2025.1708148 1 Introduction During oilfield development, as oil and gas recovery rates continue to increase, the service environment for casings becomes increasingly harsh. The downhole environment is complex and variable, encompassing multiple fluid media, while temperature and pressure conditions fluctuate continuously, resulting in dynamic changes in the metabolic activities of microbial communities.

Abstract Subsurface technologies including Carbon Capture, Utilization and Storage, geothermal systems, and hydrogen storage face persistent technical-economic barriers in monitoring precision and cost-effectiveness. Here we present a DNA sequencing method to track microbial communities in subsurface fluid flow. It addresses three main challenges: the lack of large-scale time-lapse monitoring, the absence of microbial tracer selection, and the oversight of front propagation velocity.

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