Silmon de Monerri

Abstract Urinary tract infections, caused primarily by uropathogenic E. coli, are a significant public health burden, affecting approximately 50% of women worldwide. The adhesin FimH is responsible for host receptor binding and is therefore a promising vaccine candidate, but prior studies showed that recombinant monomeric FimH is poorly immunogenic.

Citation Madhi, S. A., Anderson, A. S., Absalon, J., Radley, D., Simon, R., Jongihlati, B., Strehlau, R., van Niekerk, A. M., Izu, A., Naidoo, N., Kwatra, G., Ramsamy, Y., Said, M., Jones, S., Jose, L., Fairlie, L., Barnabas, S. L., Newton, R., Munson, S., Jefferies, Z., … Jansen, K. U. (2023). Potential for Maternally Administered Vaccine for Infant Group B Streptococcus. The New England journal of medicine, 389(3), 215–227. https://doi.org/10.1056/NEJMoa2116045

To understand the role of Ca2+ in egress, purified P. falciparum schizonts were loaded with the intracellular Ca2+ indicator, Fluo-4AM, and observed by time-lapse video microscopy as described earlier12,37. An increase in intracellular Ca2+ is observed in schizonts just before egress suggesting that Ca2+ might have a role in regulating egress of merozoites (Supplementary Movie 1; Fig. 1a). The rise in intracellular Ca2+ is crucial as P.