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Simbarashe Mabwe on Muck Rack

Simbarashe Mabwe

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Qualification of the Differential Leukocyte Count and Immunophenotyping in Cryopreserved Ex Vivo Whole Blood Assay

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By Anele Gela, Claire Imbratta, Nicole Bilek, Simbarashe Mabwe
| Wiley Online Library Verified
We developed a flow cytometry-based assay, termed Differential Leukocyte Counting and Immunophenotyping in Cryopreserved Ex vivo whole blood (DLC-ICE), that allows quantification of absolute counts and frequencies of leukocyte subsets and measures expression of activation, phenotypic and functional markers. We evaluated the performance of the DLC-ICE assay by determining inter-operator variability for processing fresh whole blood (WB) from healthy donors collected at multiple clinical sites.
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