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Simon EW

As seen in: PeerJ
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Articles by Simon EW

Transiently elevated expression of different forms of brain-derived neurotrophic factor in the neonatal medial prefrontal cortex affected anxiety and depressive-like behaviors in adolescence

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By Thomas KL, Mitchell DE, Courtney J-M, Simon EW
| PeerJ
To create an all-in-one TET-ON lentiviral vector (LV) specifically expressed in neurons, the hPGK promoter was replaced with the hSyn promoter in the plasmid pCW57-MCS1-P2A-MCS2(GFP). This was done using two-step cloning. pCW57-MCS1-P2A-MCS2(GFP) was a gift from Adam Karpf (Addgene plasmid 80924; http://n2t.net/addgene:80924; RRID:Addgene_80924).
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