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Articles

Molecular recording of cellular protein kinase activity with chemical labeling

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By De-en Sun, Siu Wang Ng, Yu Jie Zheng, Shu Xie
| biorxiv.org
Abstract Protein kinases control most cellular processes and aberrant kinase activity is involved in numerous diseases. To investigate the link between specific kinase activities and cellular phenotypes in heterogeneous cell populations and in vivo, we introduce molecular recorders of kinase activities for later analysis.
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IFNγ mediates the resistance of tumor cells to distinct NK cell subsets

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By Florian Heigwer, Tomáš Hofman, Siu Wang Ng, Irene Garcés-Lázaro
| bmj.com
Materials and methods A375 (ATCC; Cat# CRL-1619, RRID:CVCL_0037), A431 (ATCC; Cat# CRL-1555), HeLa (ATCC; Cat# CRM-CCL-2, CVCL_0030), K562 (ATCC; Cat# CCL-243, RRID:CVCL_0004), SKMel-28 (ATCC; Cat# HTB-72, RRID:CVCL_0526), MaMel-86b (RRID:CVCL_A221)68 and UKRV-Mel02 (RRID:CVCL_A700)69 cell lines were cultured in RPMI 1640 media supplemented with 10% FCS, 2 mM L-glutamine, and 1% Penicillin/Streptomycin.
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