Sofani Tafesse Gebreyesus

Introduction Click to copy section linkSection link copied! Mass spectrometry (MS)-based proteomics has advanced rapidly in both instrumentation and data analysis, enabling the quantification of thousands of proteins for various biological samples. While conventional MS approaches allow comparison of proteomic profiles across bulk samples (104–106 cells), they often obscure differences between individual cell types and fail to resolve cell-to-cell variability.

Graphical Abstract Single-Cell Phosphoproteomic Analysis In article 2402421, Hsiung-Lin Tu, Yu-Ju Chen, and co-workers present a novel phosphoproteomic chip combined with mass spectrometry to map the phosphoproteomic landscape at single-cell resolution.

1 Introduction Protein phosphorylation drives various aspects of cellular processes, such as intracellular signaling and intercellular communication.[1, 2] To date, such signal transduction information is largely acquired through ensemble-level measurements, which present limitations in unraveling the intricacies of signal flux across cellular networks and obscure the underlying heterogeneity.